Characterization of an immobilized enzyme reactor for on-line protein digestion
نویسندگان
چکیده
منابع مشابه
A Recyclable Poly(ionic liquid)s Enzyme Reactor for Highly Efficient Protein Digestion
One of the most significant tasks for proteomic research and industrial applications, is the preparation of recyclable enzyme reactor. Herein, a novel recyclable enzyme reactor has been developed based on monodispersed spherical poly(quaternary ammonium ionic liquid)s particles immobilized trypsin. A new quaternary ammonium ionic liquids functional monomer was first synthesized. The ionic l...
متن کاملEnzymatic Flow Reactor for Immobilized Enzyme Processes
Strem Chemicals, Inc. 7 Mulliken Way Newburyport, MA 01950-4098 U.S.A. Tel.: (978) 499-1600 Fax: (978) 465-3104 Email: [email protected] Strem Chemicals, Inc. 15, rue de l’Atome Zone Industrielle 67800 BISCHHEIM France Tel.: (33) 03 88 62 52 60 Fax: (33) 03 88 62 26 81 Email: [email protected] Strem Chemicals, Inc. Postfach 1215 77672 KEHL Germany Telefon: 0 78 51/ 7 58 79 Email: info.europe@s...
متن کاملEx vivo model of an immobilized-enzyme reactor.
Immobilized-enzyme reactors are beginning to be studied for a variety of therapeutic applications. To facilitate the design of these devices for different clinical situations and a diverse patient population, mathematical models may be valuable. An immobilized-heparinase (EC 4.2.2.7) reactor was selected as a model system. The device removes heparin from blood that has been anticoagulated to pr...
متن کاملDevelopment of an open-tubular trypsin reactor for on-line digestion of proteins
A study was initiated to construct a micro-reactor for protein digestion based on trypsin-coated fused-silica capillaries. Initially, surface plasmon resonance was used both for optimization of the surface chemistry applied in the preparation and for monitoring the amount of enzyme that was immobilized. The highest amount of trypsin was immobilized on dextran-coated SPR surfaces which allowed t...
متن کاملDevelopment of An Immobilized Enzyme Electrode for the Determination of Sulfite Ion
An enzyme electrode was developed using the enzyme sulfite oxidase (EC1.8.3.1) immobilized onto pig intestine. Hydrogen peroxide produced was monitored amperometrically. All experimental parameters such as pH, temperature, buffer constituent concentration, were thoroughly investigated and optimized when appropriate. The response to sulfite concentration was linear in the range 5.2´...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2016
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2016.11.021